sandwich hybridization

A method for quantification of hepatitis B Virus genome by enzyme linked solid phase sandwich hybridization

微反应板酶联夹心杂交定量检测乙型肝炎病毒基因组

Enzyme-linked sandwich hybridization in microplate for quantification of human TNF - α mRNA

微量酶联夹心杂交法定量检测TNF-αmRNA

Establishment of Sandwich Hybridization Integrated with Nuclease Protection Assay and Detection of Several HAB Species with It

双特异分子探针技术的建立及对十二种常见赤潮藻的检测

The study of identification of RSV subgroups A and B by reverse transcription polymerase chain reaction enzyme linked sandwich hybridization assay

逆转录-聚合酶链反应-酶联夹心杂交法检测呼吸道合胞病毒及其亚型感染

An enzyme-linked sandwich hybridization on microplate for quantification of hIL-18 mRNA

微板酶联夹心杂交法定量检测人IL-18mRNA

In this study , combining traditional S1 enzyme analysis with sandwich hybridization , sandwich hybridization integrated with nuclease protection assay ( NPA-SH ) was developed .

本研究利用S1酶能够保护完全匹配的双链核酸的性质，将分子生物学中的酶保护分析技术和夹心杂交技术融合在一起，创立了双特异分子探针技术以实现对赤潮藻的检测。

Objective : To develop a sensitive , specific and accurate method ( reverse transcription polymerase chain reaction enzyme linked sandwich hybridization ) for quantitative detection of human FGF mRNA .

目的：建立一种灵敏、特异、精确的定量检测人成纤维细胞生长因子mRNA（FGFmRNA）的逆转录聚合酶链反应酶联免疫吸附分析技术。

Objective : To develop a simple , sensitive , specific and accurate method based on enzyme linked sandwich hybridization on microplate for the quantitive detection of human IL 18 mRNA .

目的：建立一种高灵敏度、高特异性、精确、简便的微孔板酶联夹心杂交技术，定量检测人IL18mRNA。

Compared with previous sandwich hybridization assay , a 3 orders higher sensitivity was obtained by this method . Furthermore , this in situ signal amplification technology was readily suitable for parallel multi-target detection .

与前面几部分工作相比，本方法灵敏度提高了3个数量级，而且这种原位信号放大技术适合于在微流控芯片中进行多目标同时检测。

Total RNA extracted from the PBMC was reversely transcripted to cDNA , which used as templates for hot-start PCR amplification of IL-6 . Sandwich hybridization occurs between IL-6 amplicon , capture probe and detection probe . The hybridization signals were detected utilizing avidin-horseradish peroxidase and its substracts .

扩增产物热变性后与IL6检测探针、捕获探针进行夹心杂交，最后加入亲和素辣根过氧化物酶及其底物，显色检测杂交信号。